The purpose of this study is to examine the effect of soccer activities on body composition and physical fitness among prepubertal boys. A total of 38 prepubescent male soccer players (mean age, 12.1±0.13 years) were compared with 41 participants in control group (mean age, 12.4±0.06 years). The body composition was measured using the bio-electrical impedance analysis (BIA) method (Tanita Corp., Tokyo, Japan). 30m sprint (T30), agility T-Test, and Sit-And-Reach (S&R) Tests were performed from different motor fitness test batteries. Student’s unpaired t-test revealed that soccer and control groups were similar in age, height, body mass, BMI and lean body mass (LBM), but the control group had higher percentage of body fat (%BF) than the soccer group (p < 0.05). Also, pearson correlation analysis showed a significant moderate positive correlation between %BF and T30 (r = 0.62; p = 0.00), and a significant lower positive correlation between agility T-Test and S&R test (r = 0.35; p = 0.00, r = 0.42; p = 0.00), respectively. No significant correlation was observed between LBM, and T30, agility T-Test, and S&R test (r = –0.08; p = 0.49, r = –0.16; p = 0.15, r = –0.16; p = 0.15), respectively. In conclusion, soccer activities are associated with better physical fitness and lower BF among prepubescent boys.